Onchip Integrated Lensless Microscopy Module For Optical Monitoring Of Adherent Growing Mammalian Cells

• doi: 10.1109/IEMBS.2010.5627771
• title: On-chip integrated lensless microscopy module for optical monitoring of adherent growing mammalian cells
• publisher: IEEE
• isbn: 978-1-4244-4124-2
• issn: 1558-4615
• partnum: 10CH38122
• rank: 3184
• access_type: LOCKED
• content_type: Conferences
• abstract: Lab-on-a-chip systems are increasingly applied in cell-based assays for toxicology and drug testing. In this paper, an on-chip integrated lensless microscopy module using a direct projection method for optical monitoring of the shadow images of adherent growing mammalian cells is presented. The biological cells are conserved and interfaced by a microfabricated cavity chip with a 1 μm thick silicon nitride (Si3N4) substrate onto the surface of a 5 megapixel CMOS image sensor with 2.2 μm pixel size. The optical resolution of the assembly is estimated by the contact/proximate printing theory from optical lithography. Further characterization is made by imaging microbeads in chips with the Si3N4-membrane as well as in cavity chips with membranes made from dry film resist (DFR, thickness 20, 40 and 60 μm). The module represents a 3 × optical microscope for cell morphology imaging. The function is demonstrated by the growth monitoring of L929 cells cultured in cavity chips with Si3N4 substrate for 2 days and by checking the colorimetric staining of cells with a compromised membrane.
• article_number: 5627771
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=5627771
• html_url: https://ieeexplore.ieee.org/document/5627771/
• abstract_url: https://ieeexplore.ieee.org/document/5627771/
• publication_title: 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology
• conference_location: Buenos Aires, Argentina
• conference_dates: 31 Aug.-4 Sept. 2010
• publication_number: 5608545
• is_number: 5625939
• publication_year: 2010
• publication_date: 31 Aug.-4 Sept. 2010
• start_page: 1012
• end_page: 1015
• citing_paper_count: 3
• citing_patent_count: 7
• download_count: 398
• insert_date: 20101111
• index_terms:
  • ieee_terms:
    • Microscopy
    • Optical sensors
    • Optical imaging
    • Pixel
    • Biomedical optical imaging
    • Image resolution
  • dynamic_index_terms:
    • Mammalian Cells
    • Cell Biology
    • Cellular Biology
    • Microspheres
    • Microbeads
    • Pixel Size
    • Drug Screening
    • Drug Testing
    • Photolithography
    • Optical Lithography
    • Image Sensor
    • Camera Sensor
    • Silicon Nitride
    • Optical Resolution
    • Compromised Membrane
    • Shadow Images
    • Cell Adhesion
    • Adherent Cells
    • Microfluidic
    • Objective Lens
    • Objective Lenses
    • Microscope Objective
    • Low Magnification
    • Pixel Resolution
    • Air Gap
    • Substrate Thickness
    • Diffraction Effects
    • Diffractive Events
    • Soft Imaging System
    • Erythrosine
    • Erythrosin B
    • Sensor Plane
    • Smallest Feature
• isbn_formats:
  • format: CD, value: 978-1-4244-4124-2, isbnType: New-2005
  • format: Print ISBN, value: 978-1-4244-4123-5, isbnType: New-2005
• authors:
  • Author Name: Wei Li
    Affiliation: Department of Biohybrid Systems, Fraunhofer Institute of Biomedical Engineering, Germany
    Author URL: https://ieeexplore.ieee.org/author/37597590800
    ID: 37597590800
    Order: 1
    Author Affiliations:
    • Department of Biohybrid Systems, Fraunhofer Institute of Biomedical Engineering, Germany
  • Author Name: Thorsten Knoll
    Affiliation: Department of Microsensors & Microfludics, Fraunhofer Institute of Biomedical Engineering, Germany
    Author URL: https://ieeexplore.ieee.org/author/37587300500
    ID: 37587300500
    Order: 2
    Author Affiliations:
    • Department of Microsensors & Microfludics, Fraunhofer Institute of Biomedical Engineering, Germany
  • Author Name: Hagen Thielecke
    Affiliation: Department of Biohybrid Systems, Fraunhofer Institute of Biomedical Engineering, Saint Ingbert, Germany
    Author URL: https://ieeexplore.ieee.org/author/37370762900
    ID: 37370762900
    Order: 3
    Author Affiliations:
    • Department of Biohybrid Systems, Fraunhofer Institute of Biomedical Engineering, Saint Ingbert, Germany

Image Sensor

• sensor_type: CMOS
• resolution: 5 Megapixels (up to 1920x1080 equivalent)
• dynamic_range: Not specified
• pixel_size: 2.2 µm
• dark_current: Not specified

Optical Data

• focal_length: Not applicable
• aperture: Not applicable
• field_of_view: Not specified
• distortion: Not specified

Performance Metrics

Applications & Benefits

• cell_imaging: Used for optical monitoring and growth of adherent mammalian cells, comparable to a low magnification bright field microscope.
• benefits: Low-cost, portable, and suitable for lab-on-a-chip applications.

Supporting Organizations

• supported_by: Fraunhofer Institute for Biomedical Engineering

Manuscript Details

• publication_date: 31 Aug.-4 Sept. 2010

Relevancy Score

• score: 8
• missing_fields:
  • dynamic_range
  • dark_current
  • focal_length
  • aperture
  • field_of_view
  • distortion
  • frame_rate
  • signal_to_noise_ratio
  • sensitivity
  • shutter_speed
  • power_consumption
  • noise

Processed JSON Filename

request_13db446b-3458-451c-9353-ab9a3b0e8481-onchip_integrated_lensless_microscopy_module_for_optical_monitoring_of_adherent_growing_mammalian_cells.json