Onchip Absorption And Fluorescence Spectroscopy With Polydimethylsiloxane Pdms Microfluidic Flow Channels

• doi: 10.1109/MMB.2002.1002348
• title: On-chip absorption and fluorescence spectroscopy with polydimethylsiloxane (PDMS) microfluidic flow channels
• publisher: IEEE
• isbn: 0-7803-7480-0
• issn:
• partnum: 02EX578
• rank: 3182
• access_type: LOCKED
• content_type: Conferences
• abstract: We describe a system, which integrates PDMS microfluidic flow channels, a CMOS active pixel sensor (APS) imager, and commercial light sources for performing visible absorption and fluorescence spectroscopy. PDMS elastomer allows for spectroscopic measurements to be performed in the visible (350 to 750 nm) wavelength range. Along with a sensitive silicon photodetector such as an APS imager, measurements can be made on low concentrations of solutions by placing the microfluidic flow channels directly on the imager. Emission sources for absorption measurements are typically commercial LEDs with peak emission wavelengths that match the peak absorption wavelengths of the material under test. For calibration purposes, known dyes are tested and compared with the results obtained from the imager. Fluorescence measurements are performed in much the same way, but a band-blocking filter is placed between the microfluidic channels and the imager to greatly attenuate the pump wavelength. Typical emission sources for fluorescence are commercial Ar ion lasers and semiconductor lasers. We feel that the system has great advantages due to its miniaturized size and small fluid volumes required. Further system miniaturization is also possible due to the integration of emission sources and the expandability of CMOS imagers to integrate on board image processing.
• article_number: 1002348
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=1002348
• html_url: https://ieeexplore.ieee.org/document/1002348/
• abstract_url: https://ieeexplore.ieee.org/document/1002348/
• publication_title: 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578)
• conference_location: Madison, WI, USA
• conference_dates: 2-4 May 2002
• publication_number: 7856
• is_number: 21629
• publication_year: 2002
• publication_date: 2-4 May 2002
• start_page: 369
• end_page: 373
• citing_paper_count: 4
• citing_patent_count: 1
• download_count: 506
• insert_date: 20020807
• index_terms:
  • ieee_terms:
    • Fluorescence
    • Spectroscopy
    • Wavelength measurement
    • Electromagnetic wave absorption
    • Microfluidics
    • CMOS image sensors
    • Performance evaluation
    • Pump lasers
    • Semiconductor lasers
    • Pixel
  • dynamic_index_terms:
    • Absorption Spectroscopy
    • Absorption Spectrometry
    • Fluorescence Spectroscopy
    • Fluorescence Spectrometry
    • Excitation Spectra
    • Channel Flow
    • Microfluidic Flow Channels
    • Light Source
    • Visible Light
    • Visible Region
    • Visible Spectrum
    • Emission Sources
    • Semiconductor Laser
    • Visible Wavelength Range
    • Wavelengths In The Visible Range
    • Pump Wavelength
    • Peak Emission Wavelength
    • Sensor Pixel
    • Polydimethylsiloxane Elastomer
    • Dynamic Range
    • Measurement System
    • Array Detector
    • Optical Measurements
    • Microfluidic Device
    • Peristaltic Pump
    • Silicone Rubber
    • Silicone Elastomer
    • Tungsten Filament
    • Array Images
    • Array Tomography
    • Experimental Absorption
    • Microfluidic System
    • Dielectric Mirror
    • Bragg Mirrors
    • Avalanche Photodiode
    • Fluorescein Dye
    • Interaction Length
    • Fluorescence Wavelength
    • Incandescent Bulbs
    • Incandescent Light Bulbs
    • Incandescent Lamps
    • Tungsten Light
• isbn_formats:
  • format: Print ISBN, value: 0-7803-7480-0, isbnType: Historical
• authors:
  • Author Name: M.L. Adams
    Affiliation: Electrical Engineering and Applied Physics, California Institute of Technology, CA, USA
    Author URL: https://ieeexplore.ieee.org/author/37277443800
    ID: 37277443800
    Order: 1
    Author Affiliations:
    • Electrical Engineering and Applied Physics, California Institute of Technology, CA, USA
  • Author Name: S. Quake
    Affiliation: Electrical Engineering and Applied Physics, California Institute of Technology, CA, USA
    Author URL: https://ieeexplore.ieee.org/author/37327399800
    ID: 37327399800
    Order: 2
    Author Affiliations:
    • Electrical Engineering and Applied Physics, California Institute of Technology, CA, USA
  • Author Name: A. Scherer
    Affiliation: Electrical Engineering and Applied Physics, California Institute of Technology, CA, USA
    Author URL: https://ieeexplore.ieee.org/author/37274335900
    ID: 37274335900
    Order: 3
    Author Affiliations:
    • Electrical Engineering and Applied Physics, California Institute of Technology, CA, USA

Image Sensor

• sensor_type: CMOS
• resolution: 512x512 pixels
• dynamic_range: > 65 dB
• pixel_size: 12 um
• dark_current: > 1.3 pV/photon at room temperature

Optical Data

• focal_length: Not specified
• aperture: Not specified
• field_of_view: Not specified
• distortion: Not specified

Performance Metrics

Applications & Benefits

• cell_imaging: The system operates on small volumes and allows observation of many channels in parallel, suitable for fluorescence and absorption spectroscopy applications.
• benefits: Provides sensitive detection of low concentrations in a highly miniaturized setup.

Supporting Organizations

• supported_by: DARPA, NSF, NASA's Jet Propulsion Laboratory

Manuscript Details

• publication_date: 2-4 May 2002

Relevancy Score

• score: 10
• missing_fields:
  • focal_length
  • aperture
  • field_of_view
  • distortion
  • frame_rate
  • signal_to_noise_ratio
  • sensitivity
  • shutter_speed
  • power_consumption
  • noise

Processed JSON Filename

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