Discrimination Of Target Proteins Using Arrayed Fluorescent Liposomes Incorporated With Cholesterol By Principal Component Analysis

• doi: 10.1109/ICSENS.2015.7370493
• title: Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis
• publisher: IEEE
• isbn: 978-1-4799-8202-8
• issn:
• rank: 2304
• access_type: LOCKED
• content_type: Conferences
• abstract: A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.
• article_number: 7370493
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=7370493
• html_url: https://ieeexplore.ieee.org/document/7370493/
• abstract_url: https://ieeexplore.ieee.org/document/7370493/
• publication_title: 2015 IEEE SENSORS
• conference_location: Busan, Korea (South)
• conference_dates: 1-4 Nov. 2015
• publication_number: 7350021
• is_number: 7370096
• publication_year: 2015
• publication_date: 1-4 Nov. 2015
• start_page: 1
• end_page: 4
• citing_paper_count: 1
• citing_patent_count: 0
• download_count: 47
• insert_date: 20160107
• index_terms:
  • ieee_terms:
    • Fluorescence
    • Proteins
    • Principal component analysis
    • Arrays
    • Biomembranes
    • Lipidomics
    • Optical filters
  • author_terms:
    • Biosensor
    • Fluorescence
    • Liposome
    • Protein
    • Array
    • Cholesterol
    • Principal Component Analysis
  • dynamic_index_terms:
    • Cholesterol
    • Target Proteins
    • Concentration Of Molecules
    • Absorption Of Molecules
    • Protein Species
    • Principal Component Analysis Plot
    • Fluorescent Molecules
    • Fluorescence Intensity
    • Lysozyme
    • Array Elements
    • Optical Filter
    • Relative Fluorescence Intensity
    • Sepharose 4B
    • Liposome Preparation
    • Protein-membrane Interactions
    • Target Protein Concentration
• isbn_formats:
  • format: USB ISBN, value: 978-1-4799-8202-8, isbnType: New-2005
  • format: Electronic ISBN, value: 978-1-4799-8203-5, isbnType: New-2005
• authors:
  • Author Name: R. Imamura
    Affiliation: School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Author URL: https://ieeexplore.ieee.org/author/37085672781
    ID: 37085672781
    Order: 1
    Author Affiliations:
    • School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
  • Author Name: Z. Zhang
    Affiliation: School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Author URL: https://ieeexplore.ieee.org/author/37085518993
    ID: 37085518993
    Order: 2
    Author Affiliations:
    • School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
  • Author Name: T. Yoshikawa
    Affiliation: School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Author URL: https://ieeexplore.ieee.org/author/37085581698
    ID: 37085581698
    Order: 3
    Author Affiliations:
    • School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
  • Author Name: N. Murata
    Affiliation: School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Author URL: https://ieeexplore.ieee.org/author/37085673593
    ID: 37085673593
    Order: 4
    Author Affiliations:
    • School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
  • Author Name: K. Yamashita
    Affiliation: School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Author URL: https://ieeexplore.ieee.org/author/37379400700
    ID: 37379400700
    Order: 5
    Author Affiliations:
    • School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
  • Author Name: M. Fukuzawa
    Affiliation: School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Author URL: https://ieeexplore.ieee.org/author/37085664485
    ID: 37085664485
    Order: 6
    Author Affiliations:
    • School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
  • Author Name: M. Noda
    Affiliation: Kyoto Kogei Sen'i Daigaku, Kyoto, JP
    Author URL: https://ieeexplore.ieee.org/author/37302544700
    ID: 37302544700
    Order: 7
    Author Affiliations:
    • Kyoto Kogei Sen'i Daigaku, Kyoto, JP
  • Author Name: T. Shimanouchi
    Affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan
    Author URL: https://ieeexplore.ieee.org/author/37661828300
    ID: 37661828300
    Order: 8
    Author Affiliations:
    • Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan

Image Sensor

• sensor_type: CMOS
• resolution: not specified
• dynamic_range: not specified
• pixel_size: not specified
• dark_current: not specified

Optical Data

• focal_length: not specified
• aperture: not specified
• field_of_view: not specified
• distortion: not specified

Performance Metrics

Applications & Benefits

• cell_imaging: not specified
• benefits: not specified

Supporting Organizations

• supported_by: Japan Society for the Promotion of Science (JSPS)

Manuscript Details

• publication_date: 1-4 Nov. 2015

Relevancy Score

• score: 2
• missing_fields:
  • resolution
  • dynamic_range
  • pixel_size
  • dark_current
  • focal_length
  • aperture
  • field_of_view
  • distortion
  • frame_rate
  • signal_to_noise_ratio
  • sensitivity
  • shutter_speed
  • power_consumption
  • noise
  • cell_imaging
  • benefits

Processed JSON Filename

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