Compact Lensless Fluorescence Counting System For Single Molecular Assay

• doi: 10.1109/TBCAS.2018.2820151
• title: Compact Lensless Fluorescence Counting System for Single Molecular Assay
• publisher: IEEE
• isbn:
• issn: 1940-9990
• rank: 1963
• access_type: LOCKED
• content_type: Journals
• abstract: In digital enzyme-linked immunosorbent assay, which is used for biomarker detection and diagnosis, the concentration of target biomarkers is estimated by counting the number of fluorescence chambers in the microchamber array. We propose a compact system for counting fluorescent chambers. Our system consists of three components: a micro-reaction chamber array, an absorption filter for attenuating excitation light, and a photodetector. The absorption filter has a micro-light-pipe array (m-LPA) structure. A stacked photodiode CMOS image sensor (CIS), which can discriminate color, is applied as a photodetector. This paper describes the fabrication process enabling thin m-LPA chips. The unique low-noise characteristics of the stacked photodiode CIS that attains high sensitivity by adopting the 4T-APS configuration are explained. Furthermore, a detection method using the photobleaching phenomenon is proposed for high-sensitivity fluorescence detection. This method suggests that fluorescence by a single molecular enzyme can be detected within 30 min of the start of the fluorescence reaction.
• article_number: 8359299
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=8359299
• html_url: https://ieeexplore.ieee.org/document/8359299/
• abstract_url: https://ieeexplore.ieee.org/document/8359299/
• publication_title: IEEE Transactions on Biomedical Circuits and Systems
• conference_location:
• conference_dates:
• publication_number: 4156126
• is_number: 8500778
• publication_year: 2018
• publication_date: Oct. 2018
• start_page: 1177
• end_page: 1185
• citing_paper_count: 1
• citing_patent_count: 0
• download_count: 393
• insert_date: 20180515
• index_terms:
  • ieee_terms:
    • Photodiodes
    • Copper
    • Couplings
    • Light emitting diodes
    • Image color analysis
    • Sensor arrays
    • CMOS image sensors
  • author_terms:
    • CMOS image sensor
    • digital ELISA
    • ELISA
    • fluorescence detection
    • micro-light-pipe array
  • dynamic_index_terms:
    • Fabrication Process
    • Photodetector
    • Excitation Light
    • Light Intensity
    • Value Of Level
    • Fluorescent Light
    • Fluorescent Lamps
    • Fluorescent Tubes
    • Red Light
    • Coupling Efficiency
    • Shot Noise
    • Poisson Noise
    • Shot-noise
    • Reactive Ion Etching
    • Reactive-ion Etching
    • Prototype System
    • Reaction Chamber
    • Silicon Surface
    • Light-emitting Diode Light
    • Copper Plate
    • Excitation Light Source
    • Alignment Procedure
    • Conversion Gain
    • Photobleaching Rate
    • Fluorescein Solution
    • Manual Stage
    • Process Flow
    • Photolithography
    • Optical Lithography
    • Thermal Oxidation
    • Microbubbles
    • Electroplating
    • Electrochemical Deposition
    • Microfluidic System
• authors:
  • Author Name: Hironari Takehara
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/37074521600
    ID: 37074521600
    Order: 1
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
  • Author Name: Yuta Nakamoto
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/37086137809
    ID: 37086137809
    Order: 2
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
  • Author Name: Narihito Ikeda
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/37086487797
    ID: 37086487797
    Order: 3
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
  • Author Name: Kiyotaka Sasagawa
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/37287324600
    ID: 37287324600
    Order: 4
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
  • Author Name: Makito Haruta
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/38246962800
    ID: 38246962800
    Order: 5
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
  • Author Name: Toshihiko Noda
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/37401924800
    ID: 37401924800
    Order: 6
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
  • Author Name: Takashi Tokuda
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/37278027500
    ID: 37278027500
    Order: 7
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
  • Author Name: Jun Ohta
    Affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan
    Author URL: https://ieeexplore.ieee.org/author/37278016600
    ID: 37278016600
    Order: 8
    Author Affiliations:
    • Graduate School of Materials Science, Nara Institute of Science and Technology, Nara, Japan

Image Sensor

• sensor_type: CMOS
• resolution: Not explicitly stated
• dynamic_range: Not explicitly stated
• pixel_size: Not explicitly stated
• dark_current: Not stated; only mentioned in context of general operation metrics.

Optical Data

• focal_length: Not explicitly stated
• aperture: Not explicitly stated
• field_of_view: Not explicitly stated
• distortion: Not explicitly stated

Performance Metrics

• frame_rate: 11.88 fps
• signal_to_noise_ratio: Not explicitly stated; indicated low-noise characteristics
• sensitivity: Higher sensitivity indicated with a ratio of PD2 to PD1, specific numbers not provided
• shutter_speed: Not explicitly stated
• power_consumption: Not explicitly stated; implied low background noise during operation.
• noise: Noise level during operation was low and specified as comparable to shot noise.

Applications & Benefits

• cell_imaging: Used in digital enzyme-linked immunosorbent assay (digital ELISA) for counting single molecules in micro-reaction chambers.
• benefits: Enhanced sensitivity for detecting biomolecules at low concentrations, suitable for clinical diagnostics.

Supporting Organizations

• supported_by: Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, VLSI Design and Education Center, University of Tokyo in collaboration with Cadence Corporation and Mentor Graphics Corporation.

Manuscript Details

• publication_date: Oct. 2018

Relevancy Score

• score: 10
• missing_fields:
  • fill factor
  • quantum efficiency
  • analog-to-digital conversion techniques
  • microlenses
  • on-chip colour filters
  • global or rolling shutters
  • backside illumination

Processed JSON Filename

request_39b1777d-1fc5-4de5-bb3f-da1b2fb0562c-compact_lensless_fluorescence_counting_system_for_single_molecular_assay.json