Cmos Spectrallymultiplexed Fretonachip For Dna Analysis

• doi: 10.1109/TBCAS.2012.2230172
• title: CMOS Spectrally-Multiplexed FRET-on-a-Chip for DNA Analysis
• publisher: IEEE
• isbn:
• issn: 1940-9990
• rank: 1794
• access_type: LOCKED
• content_type: Journals
• abstract: A spectral-multiplexed fluorescence contact imaging microsystem for DNA analysis is presented. The microsystem integrates a filterless CMOS Color PhotoGate (CPG) sensor that exploits the polysilicon gate as an optical filter, and therefore does not require an external color filter. The CPG is applied to fluorescence-based transduction in a spectrally multiplexed format by differentiating among multiple emission bands, hence replacing the functionality of a bank of emission filters. A microsystem has been quantitatively modeled and prototyped based on the CPG fabricated in a standard 0.35 μm CMOS technology. The multi-color imaging capability of the microsystem in analyzing DNA targets has been validated in the detection of marker gene sequences for spinal muscular atropy disease and Escherichia coli (E. coli). Spectral-multiplexing enables the two DNA targets to be simultaneously detected with a measured detection limits of 240 nM and 210 nM for the two target concentrations at a sample volume of 10 μL for the green and red transduction channels, respectively.
• article_number: 6471258
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6471258
• html_url: https://ieeexplore.ieee.org/document/6471258/
• abstract_url: https://ieeexplore.ieee.org/document/6471258/
• publication_title: IEEE Transactions on Biomedical Circuits and Systems
• conference_location:
• conference_dates:
• publication_number: 4156126
• is_number: 6646269
• publication_year: 2013
• publication_date: Oct. 2013
• start_page: 643
• end_page: 654
• citing_paper_count: 23
• citing_patent_count: 0
• download_count: 775
• insert_date: 20130226
• index_terms:
  • ieee_terms:
    • DNA
    • CMOS integrated circuits
    • Optical sensors
    • Noise
    • Optical imaging
    • Optical device fabrication
  • author_terms:
    • Black hole quencher
    • CMOS image sensor
    • contact imaging
    • DNA detection
    • fluorescence
    • point-of-care diagnostics
    • quantum dot
    • spectral-multiplexing
  • dynamic_index_terms:
    • Deoxyribonucleic Acid
    • Deoxyribonucleic
    • Deoxyribonucleic Acid Analysis
    • Detection Limit
    • Prototype
    • Simultaneous Detection
    • Emission Band
    • Red Channel
    • Target Concentration
    • Optical Filter
    • Standard Technology
    • Muscular Diseases
    • Standard CMOS
    • Fluorescence Emission
    • Emission Intensity
    • Emission Factors
    • Incident Angle
    • Wavelength Of Light
    • Photodetector
    • Excitation Light
    • Bias Voltage
    • Förster Resonance Energy Transfer
    • Quantum Dots Emission
    • Black Hole Quencher
    • Interference Filter
    • Fluorescence-based Detection
    • Quantum Dots Solution
    • Spinal Muscular Atrophy
    • Conjugate Concentration
    • Sensor Output
• authors:
  • Author Name: Derek Ho
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/37085801412
    ID: 37085801412
    Order: 1
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
  • Author Name: M. Omair Noor
    Affiliation: Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/38559291100
    ID: 38559291100
    Order: 2
    Author Affiliations:
    • Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ONT, Canada
  • Author Name: Ulrich J. Krull
    Affiliation: Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/38255987000
    ID: 38255987000
    Order: 3
    Author Affiliations:
    • Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ONT, Canada
  • Author Name: Glenn Gulak
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/38556845900
    ID: 38556845900
    Order: 4
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
  • Author Name: Roman Genov
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/37270856200
    ID: 37270856200
    Order: 5
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada

Image Sensor

• sensor_type: CMOS Color PhotoGate (CPG) sensor
• resolution: Not explicitly mentioned, but capable of multi-color detection
• dynamic_range: Not specified, but related to CMOS technology
• pixel_size: Not specified, related to standard CMOS technology
• dark_current: Not specified in the provided text

Optical Data

• focal_length: Not mentioned
• aperture: Not mentioned
• field_of_view: Not mentioned
• distortion: Not mentioned

Performance Metrics

• frame_rate: Not mentioned
• signal_to_noise_ratio: Not explicitly mentioned, involves noise power and digital signal output based on integration time
• sensitivity: Not specified, based on quantum dot characteristics
• shutter_speed: Not mentioned
• power_consumption: Not specified, but system uses a blue LED which indicates power consideration in experimental setups
• noise: Involves multiple factors including sensor electronics and non-electronic mechanisms, but exact values not provided

Applications & Benefits

• cell_imaging: The microsystem is validated in analyzing DNA targets using fluorescence-based transduction for diagnostic applications.
• benefits: Low-cost, miniaturized design suitable for point-of-care diagnostics, allows simultaneous detection of multiple DNA targets with high sensitivity.

Supporting Organizations

• supported_by: University of Toronto, Natural Sciences and Engineering Research Council of Canada, Ontario Graduate Scholarship, and other unspecified support.

Manuscript Details

• publication_date: Oct. 2013

Relevancy Score

• score: 8
• missing_fields:
  • focal_length
  • aperture
  • field_of_view
  • distortion

Processed JSON Filename

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