Cmos Fluorescence Lifetime To Frequency Converter With Background Calibration

• doi: 10.1109/BIOCAS.2017.8325106
• title: CMOS fluorescence lifetime to frequency converter with background calibration
• publisher: IEEE
• isbn: 978-1-5090-5804-4
• issn:
• rank: 1814
• access_type: LOCKED
• content_type: Conferences
• abstract: This paper presents a novel CMOS image sensor for fluorescence lifetime measurement with direct pulse frequency modulated digital output. A parasitic insensitive multi-cycle charge modulation scheme is applied to accumulate photon-generated charges in discrete programmable time windows over multiple exposures. A built-in latched comparator generates an auto reset pulse by comparing the multicycle charge integrated output with a reference. The detected fluorescence's lifetime can be extracted by monitoring the pulse frequency variation of this auto-reset pulse signal. To compensate for the background photocurrent generated by ambient light and built-in offset, a charge pump based calibration circuitry is also proposed. Driven by a 10 KHz clock signal with 20 μs pulse width as the integration time window, the proposed circuitry can achieve 93 Hz/μW/cm2) responsivity and 19 nW/cm2 resolution at 575 nm. Applying the proposed sensor chip for lifetime measurement of a Ru(dpp)3(PF6)2 fluorescent sample, we estimate its lifetime to be 4.2 μs with 8 ns resolution.
• article_number: 8325106
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=8325106
• html_url: https://ieeexplore.ieee.org/document/8325106/
• abstract_url: https://ieeexplore.ieee.org/document/8325106/
• publication_title: 2017 IEEE Biomedical Circuits and Systems Conference (BioCAS)
• conference_location: Turin, Italy
• conference_dates: 19-21 Oct. 2017
• publication_number: 8303622
• is_number: 8325043
• publication_year: 2017
• publication_date: 19-21 Oct. 2017
• start_page: 1
• end_page: 4
• citing_paper_count: 2
• citing_patent_count: 0
• download_count: 156
• insert_date: 20180329
• index_terms:
  • ieee_terms:
    • Fluorescence
    • Charge pumps
    • Lifetime estimation
    • Calibration
    • Modulation
    • Frequency measurement
    • Photoconductivity
  • author_terms:
    • fluorescence
    • lifetime
    • charge modulation
    • cmos imager
    • charge pump
    • CTIA
  • dynamic_index_terms:
    • Fluorescence Lifetime
    • Background Calibration
    • Circuitry
    • Pulse Width
    • Pulsewidth Modulation
    • Ambient Light
    • Pulse Frequency
    • Multiple Exposures
    • Lifetime Measurements
    • Clock Signal
    • Fluorescence Lifetime Measurements
    • Charge Pump
    • Fluorescence Emission
    • Signal Propagation
    • Signal Generator
    • Nitrogen Metabolism
    • Nitrogen Cycling
    • N Cycle
    • Exponential Decay
    • Current Source
    • Current Regulations
    • Low Light
    • Excitation Light
    • Analog-to-digital Converter
    • Analog-to-digital
    • Digital-to-analog Converter
    • Digital Signal
    • Current Compensation
    • Integration Window
    • End Of Window
    • Current Sink
    • External Signals
• isbn_formats:
  • format: Print on Demand(PoD) ISBN, value: 978-1-5090-5804-4, isbnType: New-2005
  • format: USB ISBN, value: 978-1-5090-5802-0, isbnType: New-2005
  • format: Electronic ISBN, value: 978-1-5090-5803-7, isbnType: New-2005
• authors:
  • Author Name: Guoqing Fu
    Affiliation: Nano Lab, Department of Electrical and Computer Engineering, Tufts University, Medford, MA, USA
    Author URL: https://ieeexplore.ieee.org/author/37086065220
    ID: 37086065220
    Order: 1
    Author Affiliations:
    • Nano Lab, Department of Electrical and Computer Engineering, Tufts University, Medford, MA, USA
  • Author Name: Sameer Sonkusale
    Affiliation: Nano Lab, Department of Electrical and Computer Engineering, Tufts University, Medford, MA, USA
    Author URL: https://ieeexplore.ieee.org/author/37270130100
    ID: 37270130100
    Order: 2
    Author Affiliations:
    • Nano Lab, Department of Electrical and Computer Engineering, Tufts University, Medford, MA, USA

Image Sensor

• sensor_type: CMOS
• resolution: Not specified
• dynamic_range: Not specified
• pixel_size: Not specified
• dark_current: Not specified

Optical Data

• focal_length: Not specified
• aperture: Not specified
• field_of_view: Not specified
• distortion: Not specified

Performance Metrics

• power_consumption: 2 mW
• noise: 1.8 Hz (rms frequency noise)

Applications & Benefits

• cell_imaging: Fluorescence imaging, environmental monitoring, clinical chemistry, DNA sequencing, cell identification.
• benefits: Achieves low light detection with 19 nW/cm2 resolution and can digitally output pulse frequency modulated signals.

Supporting Organizations

• supported_by: Tufts University

Manuscript Details

• publication_date: 19-21 Oct. 2017

Relevancy Score

• score: 9
• missing_fields:
  • pixel_size
  • dynamic_range
  • quantum_efficiency
  • dark_current
  • signal_to_noise_ratio
  • fill_factor
  • framerate
  • sensitivity
  • readout_speed
  • temporal_noise
  • fixed-pattern_noise
  • analog_to_digital_conversion_techniques
  • microlenses
  • on_chip_colour_filters
  • global_or_rolling_shutters
  • backside_illumination

Processed JSON Filename

request_abdef6c1-3a33-482b-a5db-b392942d2318-cmos_fluorescence_lifetime_to_frequency_converter_with_background_calibration.json