A Cmosthinfilm Fluorescence Contact Imaging Microsystem For Dna Analysis

• doi: 10.1109/TCSI.2010.2043990
• title: A CMOS/Thin-Film Fluorescence Contact Imaging Microsystem for DNA Analysis
• publisher: IEEE
• isbn:
• issn: 1558-0806
• rank: 659
• access_type: LOCKED
• content_type: Journals
• abstract: A hybrid CMOS/thin-film microsystem for fluorescence contact imaging is presented. The microsystem integrates a high-performance optical interference filter and a 128 × 128 pixel active pixel sensor fabricated in a standard 0.35-¿m CMOS technology. The thin-film filter has an optical density greater than 6.0 at the wavelength of interest, providing adequate excitation rejection to the 532-nm solid-state laser. Microsystem performance is experimentally validated by imaging spots of Cyanine-3 fluorophore, conventionally used in DNA detection. The emission intensity as a function of fluorophore concentration is measured with an estimated sensitivity of 5000 fluorophore/¿m2 . A human DNA microarray has been imaged with the sensor prototype.
• article_number: 5439822
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=5439822
• html_url: https://ieeexplore.ieee.org/document/5439822/
• abstract_url: https://ieeexplore.ieee.org/document/5439822/
• publication_title: IEEE Transactions on Circuits and Systems I: Regular Papers
• conference_location:
• conference_dates:
• publication_number: 8919
• is_number: 5467096
• publication_year: 2010
• publication_date: May 2010
• start_page: 1029
• end_page: 1038
• citing_paper_count: 57
• citing_patent_count: 17
• download_count: 1103
• insert_date: 20100329
• index_terms:
  • ieee_terms:
    • Transistors
    • Fluorescence
    • DNA
    • Image analysis
    • Optical filters
    • CMOS image sensors
    • Optical imaging
    • Optical sensors
    • CMOS technology
    • Integrated optics
  • author_terms:
    • Contact imaging
    • DNA detection
    • fluorescence imaging
    • microarray
    • optical filter
  • dynamic_index_terms:
    • DNA Analysis
    • DNA Testing
    • Fluorophore
    • Fluorescent Dye
    • PCR Array
    • DNA Microarray
    • Gene Array
    • Detection Of DNA
    • Optical Filter
    • Human Microarray
    • CMOS Technology
    • Interference Filter
    • Fluorophore Concentration
    • Wavelength Of Interest
    • Light Intensity
    • Signal Processing
    • Light-emitting Diodes
    • LED Light
    • Light Emission
    • LEDs
    • Photodetector
    • Laser Light
    • Excitation Light
    • Image Sensor
    • Camera Sensor
    • Spatial Processing
    • Photomultiplier Tube
    • Temporal Processing
    • Fluorescent Dye Cy3
    • Pixel Array
    • Cy3 Dye
    • Direct Deposition
    • Contour Detection
    • Stokes Shift
    • Temporal Signal
    • Video Compression
    • Microarray Slides
    • Flicker Noise
• authors:
  • Author Name: Ritu Raj Singh
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/37954476900
    ID: 37954476900
    Order: 1
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
  • Author Name: Derek Ho
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/37085801412
    ID: 37085801412
    Order: 2
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
  • Author Name: Alireza Nilchi
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/37391383300
    ID: 37391383300
    Order: 3
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
  • Author Name: Glenn Gulak
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/38556845900
    ID: 38556845900
    Order: 4
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
  • Author Name: Patrick Yau
    Affiliation: University Health Network Microarray Centre, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/38556805700
    ID: 38556805700
    Order: 5
    Author Affiliations:
    • University Health Network Microarray Centre, Toronto, ONT, Canada
  • Author Name: Roman Genov
    Affiliation: Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada
    Author URL: https://ieeexplore.ieee.org/author/37270856200
    ID: 37270856200
    Order: 6
    Author Affiliations:
    • Department of Electrical and Computer Engineering, University of Toronto, Toronto, ONT, Canada

Image Sensor

• sensor_type: CMOS
• resolution: 128x128 pixels
• dynamic_range: Not explicitly provided
• pixel_size: Not explicitly provided
• dark_current: Not explicitly provided

Optical Data

• focal_length: Not applicable
• aperture: Not applicable
• field_of_view: Not applicable
• distortion: Not applicable

Performance Metrics

• frame_rate: Not explicitly provided
• signal_to_noise_ratio: Not explicitly provided
• sensitivity: Not explicitly provided
• shutter_speed: Not explicitly provided
• power_consumption: Not explicitly provided
• noise: Not explicitly provided

Applications & Benefits

• cell_imaging: Used for fluorescence imaging in DNA analysis of microarrays.
• benefits: Low-cost, compact, high sensitivity due to direct contact imaging.

Supporting Organizations

• supported_by: Ontario Center of Excellence (OCE), National Sciences and Engineering Research Council of Canada (NSERC)

Manuscript Details

• publication_date: May 2010

Relevancy Score

• score: 8
• missing_fields:
  • dynamic_range
  • pixel_size
  • dark_current
  • frame_rate
  • signal_to_noise_ratio
  • sensitivity
  • shutter_speed
  • power_consumption
  • noise

Processed JSON Filename

request_214358f6-0872-4f2b-abb7-486ae4262a81-a_cmosthinfilm_fluorescence_contact_imaging_microsystem_for_dna_analysis.json