A 018Spl Mum Cmos Bioluminescence Detection Labonchip

• doi: 10.1109/JSSC.2006.869785
• title: A 0.18-/spl mu/m CMOS bioluminescence detection lab-on-chip
• publisher: IEEE
• isbn:
• issn: 1558-173X
• rank: 145
• access_type: LOCKED
• content_type: Journals
• abstract: The paper describes a bioluminescence detection lab-on-chip consisting of a fiber-optic faceplate with immobilized luminescent reporters/probes that is directly coupled to an optical detection and processing CMOS system-on-chip (SoC) fabricated in a 0.18-/spl mu/m process. The lab-on-chip is customized for such applications as determining gene expression using reporter gene assays, determining intracellular ATP, and sequencing DNA. The CMOS detection SoC integrates an 8 /spl times/ 16 pixel array having the same pitch as the assay site array, a 128-channel 13-bit ADC, and column-level DSP, and is fabricated in a 0.18-/spl mu/m image sensor process. The chip is capable of detecting emission rates below 10/sup -6/ lux over 30 s of integration time at room temperature. In addition to directly coupling and matching the assay site array to the photodetector array, this low light detection is achieved by a number of techniques, including the use of very low dark current photodetectors, low-noise differential circuits, high-resolution analog-to-digital conversion, background subtraction, correlated multiple sampling, and multiple digitizations and averaging to reduce read noise. Electrical and optical characterization results as well as preliminary biological testing results are reported.
• article_number: 1599534
• pdf_url: https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=1599534
• html_url: https://ieeexplore.ieee.org/document/1599534/
• abstract_url: https://ieeexplore.ieee.org/document/1599534/
• publication_title: IEEE Journal of Solid-State Circuits
• conference_location:
• conference_dates:
• publication_number: 4
• is_number: 33637
• publication_year: 2006
• publication_date: March 2006
• start_page: 651
• end_page: 662
• citing_paper_count: 123
• citing_patent_count: 127
• download_count: 2350
• insert_date: 20060227
• index_terms:
  • ieee_terms:
    • Bioluminescence
    • Sensor arrays
    • Face detection
    • Optical coupling
    • Biomedical optical imaging
    • Optical sensors
    • CMOS process
    • Optical arrays
    • Photodetectors
    • Optical noise
  • author_terms:
    • Biosensor
    • CMOS imager
    • lab-on-chip
    • luminescence detection
  • dynamic_index_terms:
    • Bioluminescence
    • Integration Time
    • Low Detection
    • Low Light
    • Background Subtraction
    • Image Sensor
    • Camera Sensor
    • Reporter Gene Assay
    • Direct Coupling
    • Dark Current
    • Pixel Array
    • Low Dark Current
    • Dynamic Range
    • White Noise
    • Low Noise
    • Optical Path
    • Differential Signal
    • Photoemission
    • Photoelectric Effect
    • Noise Components
    • Shot Noise
    • Poisson Noise
    • CMOS Process
    • Flicker Noise
    • Shift Register
    • Differential Output
    • Differential Yield
    • Reset Voltage
    • Differential Amplifier
    • Test Chip
    • ATP Sulfurylase
    • PIN Photodiode
    • Readout Circuit
• authors:
  • Author Name: H. Eltoukhy
    Affiliation: Department of Electrical Engineering, University of Stanford, Stanford, CA, USA
    Author URL: https://ieeexplore.ieee.org/author/37425978600
    ID: 37425978600
    Order: 1
    Author Affiliations:
    • Department of Electrical Engineering, University of Stanford, Stanford, CA, USA
  • Author Name: K. Salama
    Affiliation: Department of Electrical Engineering, University of Stanford, Stanford, CA, USA
    Author URL: https://ieeexplore.ieee.org/author/37543500500
    ID: 37543500500
    Order: 2
    Author Affiliations:
    • Department of Electrical Engineering, University of Stanford, Stanford, CA, USA
  • Author Name: A.E. Gamal
    Affiliation: Department of Electrical Engineering, University of Stanford, Stanford, CA, USA
    Author URL: https://ieeexplore.ieee.org/author/37295359000
    ID: 37295359000
    Order: 3
    Author Affiliations:
    • Department of Electrical Engineering, University of Stanford, Stanford, CA, USA

Image Sensor

• sensor_type: CMOS
• resolution: 8x16 pixel array
• dynamic_range: over 100 dB
• pixel_size: unknown
• dark_current: 2.6 mV/s (not in e-/s)

Optical Data

• focal_length: not specified
• aperture: not specified
• field_of_view: not specified
• distortion: not specified

Performance Metrics

• frame_rate: at least 6 Mpixels/s
• sensitivity: near shot noise limited during integration
• power_consumption: low power to ensure reagents are not overheated
• noise: 28e- (total injected noise)

Applications & Benefits

• cell_imaging: used in bioluminescence detection assays like Pyrosequencing for DNA sequencing and gene expression assays
• benefits: capable of detecting very low light emissions down to lux range, achieved a detection sensitivity of 1.3 fmol of ATP

Supporting Organizations

• supported_by: National Institute of Health, Defense Advanced Research Projects Agency

Manuscript Details

• publication_date: March 2006

Relevancy Score

• score: 10
• missing_fields:
  • fill factor
  • quantum efficiency
  • readout speed
  • temporal noise
  • fixed-pattern noise
  • analog-to-digital conversion techniques
  • microlenses
  • on-chip colour filters
  • global or rolling shutters
  • backside illumination

Processed JSON Filename

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